The Precipitating Antigen of Streptococcus pyogenes Type 4

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T antigen of Streptococcus pyogenes: isolation and purification.

A T antigen preparation free of trypsin was obtained by application of CNBr-Sepharose linked to pure trypsin. Purification on an ion exchange chromatography column results in an electrophoretically homogeneous preparation of T protein.

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Group A streptococcus (GAS) is synonymous with Streptococcus pyogenes, the only species within this group of β-hemolytic streptococci. GAS is one of the leading pathogenic bacteria that infects children and adolescents, and is associated with a wide spectrum of infections and disease states. Worldwide, there are estimated to be >600 million cases of GAS pharyngitis (“strep throat”) and >100 mil...

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Studies on Streptococcus Pyogenes

Electron microscopic observations of Streptococcus pyogenes (strain S43, type 6) treated with trypsin and cetyltrimethylammonium bromide (Cetab) are presented. The concentration of trypsin necessary to remove the M protein antigen was shown to bring about a partial digestion of the cells. The cell protoplasm and protoplasmic membrane were affected. No microscopic changes appeared in the cell wa...

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ژورنال

عنوان ژورنال: Journal of General Microbiology

سال: 1958

ISSN: 0022-1287

DOI: 10.1099/00221287-18-1-285